快三专家指导计划 重组人UBE2D3,带His标签,Recombinant Human Ubiquitin Conjugating Enzyme E2 D3, His 生命科学产品与技术服务-生工生物工程(上海)股份有限公司 _大发快三计划_大发快三全天精准计划_快三专家指导计划
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Order NO. C620033
重组人UBE2D3,带His标签
英文名 Recombinant Human Ubiquitin Conjugating Enzyme E2 D3, His
别名 UBE2D3, His-tagged, Human
相关类别 泛素 储存 冷冻(-20℃)
分子量 Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids (a.a.) of human UBE2D3/UBC5C and 8 a.a. vector sequence including 6 × His tag at N-terminus.    
   
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10 UG 预计5日内发货
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产品描述

概述

Ubiquitin-conjugating enzyme E2 D3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2D3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2D3 is a human homolog of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. It mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBα, p105) and abnormal proteins and has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.

使用说明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Purity > 95 % by SDS-PAGE and HPLC analyses.
Source Escherichia coli.
Biological Activity Data is not available.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1mM DTT, pH 7.5.
Endotoxin Less than 1 EU/μg of rHuUBE2D3/UBC5C, His as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM
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